Application
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);Application
Figure 2:Western blot analysis using EZR mAb against human EZR (AA: 292-464) recombinant protein. (Expected MW is 47.1 kDa)Application
Figure 3:Western blot analysis using EZR mAb against HEK293 (1) and EZR (AA: 292-464)-hIgGFc transfected HEK293 (2) cell lysate.Application
Figure 4:Western blot analysis using EZR mouse mAb against MCF-7 (1), Hela (2), A431 (3), Hek293 (4), SK-N-SH (5), Jurkat (6), HepG2 (7), NIH/3T3 (8), and Cos7 (9) cell lysate.Application
Figure 5:Immunofluorescence analysis of A549 cells using EZR mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)Application
Figure 6:Flow cytometric analysis of MCF-7 cells using EZR mouse mAb (green) and negative control (red).Application
Figure 7:Flow cytometric analysis of Hela cells using EZR mouse mAb (green) and negative control (red).| Product Name | EZR |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CVL; CVIL; VIL2; HEL-S-105 |
| Immunogen | Purified recombinant fragment of human EZR (AA: 292-464) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Unknow |
| Tested Applications | WBICCFCMICC/IF |
| WB:1:250~1:1000 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseMonkey |
| Alternative Names | CVL CVIL VIL2 HEL-S-105 |
|---|---|
| Molecular Weight(MW) | 69.4kDa |
| Entrez Gene | 7430 |
|---|
Application
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Application
Figure 2:Western blot analysis using EZR mAb against human EZR (AA: 292-464) recombinant protein. (Expected MW is 47.1 kDa)
Application
Figure 3:Western blot analysis using EZR mAb against HEK293 (1) and EZR (AA: 292-464)-hIgGFc transfected HEK293 (2) cell lysate.
Application
Figure 4:Western blot analysis using EZR mouse mAb against MCF-7 (1), Hela (2), A431 (3), Hek293 (4), SK-N-SH (5), Jurkat (6), HepG2 (7), NIH/3T3 (8), and Cos7 (9) cell lysate.
Application
Figure 5:Immunofluorescence analysis of A549 cells using EZR mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Application
Figure 6:Flow cytometric analysis of MCF-7 cells using EZR mouse mAb (green) and negative control (red).
Application
Figure 7:Flow cytometric analysis of Hela cells using EZR mouse mAb (green) and negative control (red).| Application Notes | WB:1:250~1:1000 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Purified antibody in PBS with 0.05% sodium azide |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
