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CSF3

Catalog Number:OM640187
Human
WB;IHC;ICC;ICC/IF
OM640187
Discount Price
OM640187-1.0mg/ml
1~2 Week
1.0mg/ml
1~2 Week
Product Profile
Product Name CSF3
Antibody Type Primary Antibodies
Antigen Alias GCSF; CSF3OS; C17orf33
Immunogen Purified recombinant fragment of human CSF3 (AA: 1-207) expressed in E. Coli.
Key Feature
Clonality Monoclonal
Host Species Unknow
Tested Applications WBIHCICCICC/IF
WB:1:250~1:1000
IHC:1:20~1:100
ICC:1:100~1:200
ICC/IF:1:100~1:200
Notes:Optimal dilutions/concentrations should be determined by the researcher.
Species Reactivity Human
Target Information
Alternative Names GCSF
CSF3OS
C17orf33
Molecular Weight(MW) 22.3kDa
Database Links
Entrez Gene 1440
Application

Application

Figure 1:Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

Application

Figure 2:Western blot analysis using CSF3 mAb against human CSF3 (AA: 1-207) recombinant protein. (Expected MW is 48.2 kDa)

Application

Figure 3:Western blot analysis using CSF3 mAb against HEK293 (1) and CSF3 (AA: 1-207)-hIgGFc transfected HEK293 (2) cell lysate.

Application

Figure 4:Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using CSF3 mouse mAb with DAB staining.

Application

Figure 4:Immunofluorescence analysis of A549 cells using CSF3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Application

Figure 5:Immunofluorescence analysis of Hela cells using CSF3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Application Notes WB:1:250~1:1000
IHC:1:20~1:100
ICC:1:100~1:200
ICC/IF:1:100~1:200
Notes:Optimal dilutions/concentrations should be determined by the researcher.
Additional Information
Form Purified antibody in PBS with 0.05% sodium azide
Storage Instructions 4℃; -20℃ for long term storage
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