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CD6

Catalog Number:OM640203
Human
WB;IHC;ICC;FCM;ICC/IF
OM640203
Discount Price
OM640203-1.0mg/ml
1~2 Week
1.0mg/ml
1~2 Week
Product Profile
Product Name CD6
Antibody Type Primary Antibodies
Antigen Alias TP120; FLJ44171
Immunogen Purified recombinant fragment of human CD6 (AA: 18-199) expressed in E. Coli.
Key Feature
Clonality Monoclonal
Host Species Unknow
Tested Applications WBIHCICCFCMICC/IF
WB:1:250~1:1000
IHC:1:20~1:100
ICC:1:100~1:200
ICC/IF:1:100~1:200
Notes:Optimal dilutions/concentrations should be determined by the researcher.
Species Reactivity Human
Target Information
Alternative Names TP120
FLJ44171
Molecular Weight(MW) 71.8kDa
Database Links
Entrez Gene 923
Application

Application

Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

Application

Figure 1: Western blot analysis using CD6 mAb against human CD6(AA: 18-199) recombinant protein. (Expected MW is 44.8 kDa)

Application

Figure 2: Western blot analysis using CD6 mAb against HEK293 (1) and CD6 (AA: 18-199)-hIgGFc transfected HEK293 (2) cell lysate.

Application

Figure 3:Immunofluorescence analysis of Hela cells using CD6 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Application

Figure 4: Flow cytometric analysis of Jurkat cells using CD6 mouse mAb (green) and negative control (red).

Application

Figure 5: Immunohistochemical analysis of paraffin-embedded tissues using breast cancer mouse mAb with DAB staining.

Application

Figure 6: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using rectum cancer mouse mAb with DAB staining.
Application Notes WB:1:250~1:1000
IHC:1:20~1:100
ICC:1:100~1:200
ICC/IF:1:100~1:200
Notes:Optimal dilutions/concentrations should be determined by the researcher.
Additional Information
Form Purified antibody in PBS with 0.05% sodium azide
Storage Instructions 4℃; -20℃ for long term storage
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