Application
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);Application
Figure 1: Western blot analysis using PDGFRA mAb against human PDGFRA(AA: 361-528) recombinant protein. (Expected MW is 44.8 kDa)Application
Figure 2: Western blot analysis using PDGFRA mAb against HEK293 (1) and PDGFRA(AA: 361-528)-hIgGFc transfected HEK293 (2) cell lysate.Application
Figure 3:Immunofluorescence analysis of Hela cells using PDGFRA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)| Product Name | PDGFRA |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD140A; PDGFR2; PDGFR-2; RHEPDGFRA |
| Immunogen | Purified recombinant fragment of human PDGFRA (AA: 361-528) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Unknow |
| Tested Applications | WBICCICC/IF |
| WB:1:250~1:1000 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Alternative Names | CD140A PDGFR2 PDGFR-2 RHEPDGFRA |
|---|---|
| Molecular Weight(MW) | 122.7kDa |
| Entrez Gene | 5156 |
|---|
Application
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Application
Figure 1: Western blot analysis using PDGFRA mAb against human PDGFRA(AA: 361-528) recombinant protein. (Expected MW is 44.8 kDa)
Application
Figure 2: Western blot analysis using PDGFRA mAb against HEK293 (1) and PDGFRA(AA: 361-528)-hIgGFc transfected HEK293 (2) cell lysate.
Application
Figure 3:Immunofluorescence analysis of Hela cells using PDGFRA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)| Application Notes | WB:1:250~1:1000 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Purified antibody from tissue culture in PBS with 0.05% sodium azide |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
