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Black line: Control Antigen (100 ng);
Purple line: Antigen(10ng);
Blue line: Antigen (50 ng);
Red line: Antigen (100 ng);
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Figure 1: Western blot analysis using PKHD1 mAb against mouse PKHD1(AA: 3878-4060) recombinant protein. (Expected MW is 23 kDa)
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Figure 2:Immunofluorescence analysis of A431 cells using PKHD1 Rat mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
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Figure 3: Flow cytometric analysis of Hela cells using PKHD1 Rat mAb (green) and negative control (red).
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Figure 4: Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using PKHD1 Rat mAb with DAB staining.