Application
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);Application
Figure 1: Western blot analysis using EIF5A mAb against human EIF5A (AA: full(1-154)) recombinant protein. (Expected MW is 42.8 kDa)Application
Figure 2: Western blot analysis using EIF5A mAb against HEK293 (1) and EIF5A (AA: full(1-154))-hIgGFc transfected HEK293 (2) cell lysate.Application
Figure 3:Immunofluorescence analysis of Hela cells using EIF5A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)Application
Figure 4: Flow cytometric analysis of Hela cells using EIF5A mouse mAb (green) and negative control (red).Application
Figure 5: Immunohistochemical analysis of paraffin-embedded colon cancer tissues using EIF5A mouse mAb with DAB staining.Application
Figure 6: Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using EIF5A mouse mAb with DAB staining.| Product Name | EIF5A |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EIF-5A; EIF5A1; eIF5AI |
| Immunogen | Purified recombinant fragment of human EIF5A (AA: full(1-154)) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Unknow |
| Tested Applications | WBIHCICCFCMICC/IF |
| WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Alternative Names | EIF-5A EIF5A1 eIF5AI |
|---|---|
| Molecular Weight(MW) | 16.8kDa |
| Entrez Gene | 1984 |
|---|
Application
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Application
Figure 1: Western blot analysis using EIF5A mAb against human EIF5A (AA: full(1-154)) recombinant protein. (Expected MW is 42.8 kDa)
Application
Figure 2: Western blot analysis using EIF5A mAb against HEK293 (1) and EIF5A (AA: full(1-154))-hIgGFc transfected HEK293 (2) cell lysate.
Application
Figure 3:Immunofluorescence analysis of Hela cells using EIF5A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Application
Figure 4: Flow cytometric analysis of Hela cells using EIF5A mouse mAb (green) and negative control (red).
Application
Figure 5: Immunohistochemical analysis of paraffin-embedded colon cancer tissues using EIF5A mouse mAb with DAB staining.
Application
Figure 6: Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using EIF5A mouse mAb with DAB staining.| Application Notes | WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Purified antibody from tissue culture in PBS with 0.05% sodium azide |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
