Application
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);Application
Figure 1: Western blot analysis using CDH16 mAb against human CDH16 (AA: EX(558-721)) recombinant protein. (Expected MW is 43.6 kDa)Application
Figure 2: Western blot analysis using CDH16 mAb against HEK293 (1) and CDH16 (AA: EX(558-721))-hIgGFc transfected HEK293 (2) cell lysate.Application
Figure 3: Western blot analysis using CDH16 mouse mAb against A431 (1), Jurkat (2) cell lysate.Application
Figure 4: Immunofluorescence analysis of A431 cells using CDH16 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)Application
Figure 5: Flow cytometric analysis of A431 cells using CDH16 mouse mAb (green) and negative control (red).Application
Figure 6: Immunohistochemical analysis of paraffin-embedded kidney tissues using CDH16 mouse mAb with DAB staining.| Product Name | CDH16 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | KSP-Cadherin |
| Immunogen | Purified recombinant fragment of human CDH16 (AA: EX(558-721)) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Unknow |
| Tested Applications | WBIHCICCFCMICC/IF |
| WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Alternative Names | KSP-Cadherin |
|---|---|
| Molecular Weight(MW) | 90kDa |
| Entrez Gene | 1014 |
|---|
Application
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Application
Figure 1: Western blot analysis using CDH16 mAb against human CDH16 (AA: EX(558-721)) recombinant protein. (Expected MW is 43.6 kDa)
Application
Figure 2: Western blot analysis using CDH16 mAb against HEK293 (1) and CDH16 (AA: EX(558-721))-hIgGFc transfected HEK293 (2) cell lysate.
Application
Figure 3: Western blot analysis using CDH16 mouse mAb against A431 (1), Jurkat (2) cell lysate.
Application
Figure 4: Immunofluorescence analysis of A431 cells using CDH16 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Application
Figure 5: Flow cytometric analysis of A431 cells using CDH16 mouse mAb (green) and negative control (red).
Application
Figure 6: Immunohistochemical analysis of paraffin-embedded kidney tissues using CDH16 mouse mAb with DAB staining.| Application Notes | WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Purified antibody from tissue culture in PBS with 0.05% sodium azide |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
