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EGFR mutant

Catalog Number:OM640439
Human
WB;IHC;FCM;ICC/IF
OM640439
Discount Price
OM640439-1.0mg/ml
1~2 Week
1.0mg/ml
1~2 Week
Product Profile
Product Name EGFR mutant
Antibody Type Primary Antibodies
Antigen Alias ERBB; HER1; mENA; ERBB1; PIG61
Immunogen Purified recombinant fragment of human EGFR mutant (AA: 693-893) expressed in E. Coli.
Key Feature
Clonality Monoclonal
Host Species Unknow
Tested Applications WBIHCFCMICC/IF
WB:1:250~1:1000
IHC:1:20~1:100
ICC/IF:1:100~1:200
Notes:Optimal dilutions/concentrations should be determined by the researcher.
Species Reactivity Human
Target Information
Alternative Names ERBB
HER1
mENA
ERBB1
PIG61
Molecular Weight(MW) 175kDa
Database Links
Entrez Gene 1956
Application

Application

Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

Application

Figure 1: Western blot analysis using EGFR mutant mAb against human EGFR mutant recombinant protein. (Expected MW is 36.9 kDa)

Application

Figure 2: Western blot analysis using EGFR mutant mAb against HEK293 (1) and EGFR mutant (AA: 693-893)-hIgGFc transfected HEK293 (2) cell lysate.

Application

Figure 3: Western blot analysis using EGFR mutant mouse mAb against SPC-A-1 (1), A549 (2), HepG2 (3) and MCF-7 (4) cell lysate.

Application

Figure 4: Flow cytometric analysis of HepG2 cells using EGFR mutant mouse mAb (green) and negative control (purple).

Application

Figure 5: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using EGFR mutant mouse mAb with DAB staining.

Application

Figure 6: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using EGFR mutant mouse mAb with DAB staining.
Application Notes WB:1:250~1:1000
IHC:1:20~1:100
ICC/IF:1:100~1:200
Notes:Optimal dilutions/concentrations should be determined by the researcher.
Additional Information
Form Purified antibody in PBS with 0.05% sodium azide
Storage Instructions 4℃; -20℃ for long term storage
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