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Black line: Control Antigen (100 ng);
Purple line: Antigen(10ng);
Blue line: Antigen (50 ng);
Red line: Antigen (100 ng);
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Figure 1: Western blot analysis using EGFR mutant mAb against human EGFR mutant recombinant protein. (Expected MW is 36.9 kDa)
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Figure 2: Western blot analysis using EGFR mutant mAb against HEK293 (1) and EGFR mutant (AA: 693-893)-hIgGFc transfected HEK293 (2) cell lysate.
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Figure 3: Western blot analysis using EGFR mutant mouse mAb against SPC-A-1 (1), A549 (2), HepG2 (3) and MCF-7 (4) cell lysate.
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Figure 4: Flow cytometric analysis of HepG2 cells using EGFR mutant mouse mAb (green) and negative control (purple).
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Figure 5: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using EGFR mutant mouse mAb with DAB staining.
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Figure 6: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using EGFR mutant mouse mAb with DAB staining.