Application
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);Application
Figure 1: Western blot analysis using IL3RA mAb against human IL3RA recombinant protein. (Expected MW is 38.3 kDa)Application
Figure 2: Western blot analysis using IL3RA mAb against HEK293 (1) and IL3RA (AA: 200-305)-hIgGFc transfected HEK293 (2) cell lysate.Application
Figure 3: Immunofluorescence analysis of Hela cells using IL3RA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Application
Figure 4: Flow cytometric analysis of Hela cells using IL3RA mouse mAb (green) and negative control (red).| Product Name | IL3RA |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IL3R; CD123; IL3RX; IL3RY; IL3RAY; hIL-3Ra |
| Immunogen | Purified recombinant fragment of human IL3RA (AA: 200-305) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Unknow |
| Tested Applications | WBICCFCMICC/IF |
| WB:1:250~1:1000 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Alternative Names | IL3R CD123 IL3RX IL3RY IL3RAY hIL-3Ra |
|---|---|
| Molecular Weight(MW) | 43.3kDa |
| Entrez Gene | 3563 |
|---|
Application
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Application
Figure 1: Western blot analysis using IL3RA mAb against human IL3RA recombinant protein. (Expected MW is 38.3 kDa)
Application
Figure 2: Western blot analysis using IL3RA mAb against HEK293 (1) and IL3RA (AA: 200-305)-hIgGFc transfected HEK293 (2) cell lysate.
Application
Figure 3: Immunofluorescence analysis of Hela cells using IL3RA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Application
Figure 4: Flow cytometric analysis of Hela cells using IL3RA mouse mAb (green) and negative control (red).| Application Notes | WB:1:250~1:1000 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Ascitic fluid containing 0.03% sodium azide. |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
