Application
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);Application
Figure 1: Western blot analysis using MAPK14 mAb against human MAPK14 recombinant protein. (Expected MW is 33 kDa)Application
Figure 2: Western blot analysis using MAPK14 mAb against HEK293 (1) and MAPK14 (AA: 299-360)-hIgGFc transfected HEK293 (2) cell lysate.Application
Figure 3: Western blot analysis using MAPK14 mouse mAb against HeLa (1), NIH/3T3 (2), Jurkat (3), Raw264.7 (4), PC-12 (5), C6 (6) and COS7 (7) cell lysate.Application
Figure 4: Immunofluorescence analysis of HeLa cells using MAPK14 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Application
Figure 5: Flow cytometric analysis of HeLa cells using MAPK14 mouse mAb (green) and negative control (red).Application
Figure 6: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using MAPK14 mouse mAb with DAB staining.Application
Figure 7: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using MAPK14 mouse mAb with DAB staining.| Product Name | MAPK14 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RK; p38; CSBP; EXIP; Mxi2; CSBP1; CSBP2; CSPB1; PRKM14; PRKM15; SAPK2A; p38ALPHA |
| Immunogen | Purified recombinant fragment of human MAPK14 (AA: 299-360) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Unknow |
| Tested Applications | WBIHCICCFCMICC/IF |
| WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Alternative Names | RK p38 CSBP EXIP Mxi2 CSBP1 CSBP2 CSPB1 PRKM14 PRKM15 SAPK2A p38ALPHA |
|---|---|
| Molecular Weight(MW) | 41.3kDa |
| Entrez Gene | 1432 |
|---|
Application
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Application
Figure 1: Western blot analysis using MAPK14 mAb against human MAPK14 recombinant protein. (Expected MW is 33 kDa)
Application
Figure 2: Western blot analysis using MAPK14 mAb against HEK293 (1) and MAPK14 (AA: 299-360)-hIgGFc transfected HEK293 (2) cell lysate.
Application
Figure 3: Western blot analysis using MAPK14 mouse mAb against HeLa (1), NIH/3T3 (2), Jurkat (3), Raw264.7 (4), PC-12 (5), C6 (6) and COS7 (7) cell lysate.
Application
Figure 4: Immunofluorescence analysis of HeLa cells using MAPK14 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Application
Figure 5: Flow cytometric analysis of HeLa cells using MAPK14 mouse mAb (green) and negative control (red).
Application
Figure 6: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using MAPK14 mouse mAb with DAB staining.
Application
Figure 7: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using MAPK14 mouse mAb with DAB staining.| Application Notes | WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Purified antibody in PBS with 0.05% sodium azide |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
