Application
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);Application
Figure 1: Western blot analysis using NT5E mAb against human NT5E (AA: 30-250) recombinant protein. (Expected MW is 26.6 kDa)Application
Figure 2: Western blot analysis using NT5E mAb against HEK293 (1) and NT5E (AA: 30-250)-hIgGFc transfected HEK293 (2) cell lysate.Application
Figure 3: Western blot analysis using NT5E mouse mAb against A431 cell lysate.Application
Figure 4: Flow cytometric analysis of A431 cells using NT5E mouse mAb (green) and negative control (red).Application
Figure 5: Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues using NT5E mouse mAb with DAB staining.Application
Figure 6: Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using NT5E mouse mAb with DAB staining.| Product Name | NT5E |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NT; eN; NT5; NTE; eNT; CD73; E5NT; CALJA |
| Immunogen | Purified recombinant fragment of human NT5E (AA: 30-250) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Unknow |
| Tested Applications | WBIHCFCMICC/IF |
| WB:1:250~1:1000 IHC:1:20~1:100 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Alternative Names | NT eN NT5 NTE eNT CD73 E5NT CALJA |
|---|---|
| Molecular Weight(MW) | 63.4kDa |
| Entrez Gene | 4907 |
|---|
Application
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Application
Figure 1: Western blot analysis using NT5E mAb against human NT5E (AA: 30-250) recombinant protein. (Expected MW is 26.6 kDa)
Application
Figure 2: Western blot analysis using NT5E mAb against HEK293 (1) and NT5E (AA: 30-250)-hIgGFc transfected HEK293 (2) cell lysate.
Application
Figure 3: Western blot analysis using NT5E mouse mAb against A431 cell lysate.
Application
Figure 4: Flow cytometric analysis of A431 cells using NT5E mouse mAb (green) and negative control (red).
Application
Figure 5: Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues using NT5E mouse mAb with DAB staining.
Application
Figure 6: Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using NT5E mouse mAb with DAB staining.| Application Notes | WB:1:250~1:1000 IHC:1:20~1:100 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Purified antibody from tissue culture in PBS with 0.05% sodium azide |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
