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Black line: Control Antigen (100 ng);
Purple line: Antigen(10ng);
Blue line: Antigen (50 ng);
Red line: Antigen (100 ng);
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Figure 1: Western blot analysis using PER3 mAb against human PER3 (AA: 723-954) recombinant protein. (Expected MW is 50.7 kDa)
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Figure 2: Western blot analysis using PER3 mAb against HEK293 (1) and PER3 (AA: 723-954)-hIgGFc transfected HEK293 (2) cell lysate.
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Figure 3:Immunofluorescence analysis of Hela cells using PER3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
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Figure 4: Flow cytometric analysis of Hela cells using PER3 mouse mAb (green) and negative control (red).
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Figure 5: Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using PER3 mouse mAb with DAB staining.
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Figure 6: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using PER3 mouse mAb with DAB staining.