Application
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);Application
Figure 1: Western blot analysis using RB1 mAb against human RB1 (AA: 2106-2784) recombinant protein. (Expected MW is 38.8 kDa)Application
Figure 2: Western blot analysis using RB1 mouse mAb against Jurkat (1) and A431 (2) cell lysate.Application
Figure 3: Immunofluorescence analysis of NIH/3T3 cells using RB1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)Application
Figure 4: Flow cytometric analysis of Jurkat cells using RB1 mouse mAb (green) and negative control (red).| Product Name | RB1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RB; pRb; OSRC; pp110; p105-Rb |
| Immunogen | Purified recombinant fragment of human RB1 (AA: 2106-2784) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Unknow |
| Tested Applications | WBICCFCMICC/IF |
| WB:1:250~1:1000 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Alternative Names | RB pRb OSRC pp110 p105-Rb |
|---|---|
| Molecular Weight(MW) | 106.2kDa |
| Entrez Gene | 5925 |
|---|
Application
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Application
Figure 1: Western blot analysis using RB1 mAb against human RB1 (AA: 2106-2784) recombinant protein. (Expected MW is 38.8 kDa)
Application
Figure 2: Western blot analysis using RB1 mouse mAb against Jurkat (1) and A431 (2) cell lysate.
Application
Figure 3: Immunofluorescence analysis of NIH/3T3 cells using RB1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Application
Figure 4: Flow cytometric analysis of Jurkat cells using RB1 mouse mAb (green) and negative control (red).| Application Notes | WB:1:250~1:1000 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Purified antibody in PBS with 0.05% sodium azide |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
