Application
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);Application
Figure 1: Western blot analysis using RPS6KA2 mAb against human RPS6KA2 (AA: 415-734) recombinant protein. (Expected MW is 62 kDa)Application
Figure 2: Western blot analysis using RPS6KA2 mAb against HEK293 (1) and RPS6KA2 (AA: 415-734)-hIgGFc transfected HEK293 (2) cell lysate.Application
Figure 3: Western blot analysis using RPS6KA2 mouse mAb against Hela (1), A431 (2), HEK293 (3), Jurkat (4), HepG2 (5), MCF-7 (6), NIH/3T3 (7) cell lysate.Application
Figure 4:Immunofluorescence analysis of A431 cells using RPS6KA2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)Application
Figure 5: Flow cytometric analysis of Hela cells using RPS6KA2 mouse mAb (green) and negative control (red).Application
Figure 6: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using RPS6KA2 mouse mAb with DAB staining.Application
Figure 7: Immunohistochemical analysis of paraffin-embedded lung cancer tissues using RPS6KA2 mouse mAb with DAB staining.| Product Name | RPS6KA2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RSK; HU-2; RSK3; p90-RSK3; pp90RSK3; MAPKAPK1C; S6K-alpha; S6K-alpha2 |
| Immunogen | Purified recombinant fragment of human RPS6KA2 (AA: 415-734) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Unknow |
| Tested Applications | WBIHCICCFCMICC/IF |
| WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouse |
| Alternative Names | RSK HU-2 RSK3 p90-RSK3 pp90RSK3 MAPKAPK1C S6K-alpha S6K-alpha2 |
|---|---|
| Molecular Weight(MW) | 83.2kDa |
| Entrez Gene | 6196 |
|---|
Application
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Application
Figure 1: Western blot analysis using RPS6KA2 mAb against human RPS6KA2 (AA: 415-734) recombinant protein. (Expected MW is 62 kDa)
Application
Figure 2: Western blot analysis using RPS6KA2 mAb against HEK293 (1) and RPS6KA2 (AA: 415-734)-hIgGFc transfected HEK293 (2) cell lysate.
Application
Figure 3: Western blot analysis using RPS6KA2 mouse mAb against Hela (1), A431 (2), HEK293 (3), Jurkat (4), HepG2 (5), MCF-7 (6), NIH/3T3 (7) cell lysate.
Application
Figure 4:Immunofluorescence analysis of A431 cells using RPS6KA2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Application
Figure 5: Flow cytometric analysis of Hela cells using RPS6KA2 mouse mAb (green) and negative control (red).
Application
Figure 6: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using RPS6KA2 mouse mAb with DAB staining.
Application
Figure 7: Immunohistochemical analysis of paraffin-embedded lung cancer tissues using RPS6KA2 mouse mAb with DAB staining.| Application Notes | WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Purified antibody in PBS with 0.05% sodium azide. |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
