Application
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);Application
Figure 1: Western blot analysis using SDC1 mAb against human SDC1 (AA: 28-171) recombinant protein. (Expected MW is 44.4 kDa)Application
Figure 2: Western blot analysis using SDC1 mAb against HEK293 (1) and SDC1 (AA: 28-171)-hIgGFc transfected HEK293 (2) cell lysate.Application
Figure 3: Western blot analysis using SDC1 mouse mAb against Hela (1), MCF-7 (2), HepG2 (3), T47D (4), Jurkat (5), NIH/3T3 (6) cell lysate.Application
Figure 4: Immunofluorescence analysis of HepG2 cells using SDC1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Secondary antibody from Fisher (Cat#: 35503)Application
Figure 5: Flow cytometric analysis of A431 cells using SDC1 mouse mAb (green) and negative control (red).| Product Name | SDC1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SDC; CD138; SYND1; syndecan |
| Immunogen | Purified recombinant fragment of human SDC1 (AA: 28-171) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Unknow |
| Tested Applications | WBICCFCMICC/IF |
| WB:1:250~1:1000 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouse |
| Alternative Names | SDC CD138 SYND1 syndecan |
|---|---|
| Molecular Weight(MW) | 32.5kDa |
| Entrez Gene | 6382 |
|---|
Application
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Application
Figure 1: Western blot analysis using SDC1 mAb against human SDC1 (AA: 28-171) recombinant protein. (Expected MW is 44.4 kDa)
Application
Figure 2: Western blot analysis using SDC1 mAb against HEK293 (1) and SDC1 (AA: 28-171)-hIgGFc transfected HEK293 (2) cell lysate.
Application
Figure 3: Western blot analysis using SDC1 mouse mAb against Hela (1), MCF-7 (2), HepG2 (3), T47D (4), Jurkat (5), NIH/3T3 (6) cell lysate.
Application
Figure 4: Immunofluorescence analysis of HepG2 cells using SDC1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Secondary antibody from Fisher (Cat#: 35503)
Application
Figure 5: Flow cytometric analysis of A431 cells using SDC1 mouse mAb (green) and negative control (red).| Application Notes | WB:1:250~1:1000 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Purified antibody in PBS with 0.05% sodium azide. |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
