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TWIST1

Catalog Number:OM640636
Human;Mouse
WB;IHC;ICC;FCM;ICC/IF
OM640636
Discount Price
OM640636-1.0mg/ml
1~2 Week
1.0mg/ml
1~2 Week
Product Profile
Product Name TWIST1
Antibody Type Primary Antibodies
Antigen Alias SCS; ACS3; CRS1; BPES2; BPES3; TWIST; bHLHa38
Immunogen Purified recombinant fragment of human TWIST1 (AA: 9-74) expressed in E. Coli.
Key Feature
Clonality Monoclonal
Host Species Unknow
Tested Applications WBIHCICCFCMICC/IF
WB:1:250~1:1000
IHC:1:20~1:100
ICC:1:100~1:200
ICC/IF:1:100~1:200
Notes:Optimal dilutions/concentrations should be determined by the researcher.
Species Reactivity HumanMouse
Target Information
Alternative Names SCS
ACS3
CRS1
BPES2
BPES3
TWIST
bHLHa38
Molecular Weight(MW) 21kDa
Database Links
Entrez Gene 7291
Application

Application

Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

Application

Figure 1: Western blot analysis using TWIST1 mAb against human TWIST1 recombinant protein. (Expected MW is 31.9 kDa)

Application

Figure 2: Western blot analysis using TWIST1 mAb against HEK293 (1) and TWIST1 (AA: 9-74)-hIgGFc transfected HEK293 (2) cell lysate.

Application

Figure 3: Western blot analysis using TWIST1 mouse mAb against NIH/3T3 (1), JURKAT (2), HELA (3), A549 (4), RAJI (5) and OCM-1 (6) cell lysate.

Application

Figure 4: Immunofluorescence analysis of Hela cells using TWIST1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.

Application

Figure 5: Flow cytometric analysis of Hela cells using TWIST1 mouse mAb (green) and negative control (red).

Application

Figure 6: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using TWIST1 mouse mAb with DAB staining.

Application

Figure 7: Immunohistochemical analysis of paraffin-embedded colon cancer tissues using TWIST1 mouse mAb with DAB staining.
Application Notes WB:1:250~1:1000
IHC:1:20~1:100
ICC:1:100~1:200
ICC/IF:1:100~1:200
Notes:Optimal dilutions/concentrations should be determined by the researcher.
Additional Information
Form Purified antibody in PBS with 0.05% sodium azide
Storage Instructions 4℃; -20℃ for long term storage
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