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Black line: Control Antigen (100 ng);
Purple line: Antigen(10ng);
Blue line: Antigen (50 ng);
Red line: Antigen (100 ng);
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Figure 1: Western blot analysis using TWIST1 mAb against human TWIST1 recombinant protein. (Expected MW is 31.9 kDa)
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Figure 2: Western blot analysis using TWIST1 mAb against HEK293 (1) and TWIST1 (AA: 9-74)-hIgGFc transfected HEK293 (2) cell lysate.
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Figure 3: Western blot analysis using TWIST1 mouse mAb against NIH/3T3 (1), JURKAT (2), HELA (3), A549 (4), RAJI (5) and OCM-1 (6) cell lysate.
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Figure 4: Immunofluorescence analysis of Hela cells using TWIST1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
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Figure 5: Flow cytometric analysis of Hela cells using TWIST1 mouse mAb (green) and negative control (red).
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Figure 6: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using TWIST1 mouse mAb with DAB staining.
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Figure 7: Immunohistochemical analysis of paraffin-embedded colon cancer tissues using TWIST1 mouse mAb with DAB staining.