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XRCC6

Catalog Number:OM640646
Human
WB;IHC;ICC;FCM;ICC/IF
OM640646
Discount Price
OM640646-1.0mg/ml
1~2 Week
1.0mg/ml
1~2 Week
Product Profile
Product Name XRCC6
Antibody Type Primary Antibodies
Antigen Alias ML8; KU70; TLAA; CTC75; CTCBF; G22P1
Immunogen Purified recombinant fragment of human XRCC6 (AA: 6-214) expressed in E. Coli.
Key Feature
Clonality Monoclonal
Host Species Unknow
Tested Applications WBIHCICCFCMICC/IF
WB:1:250~1:1000
IHC:1:20~1:100
ICC:1:100~1:200
ICC/IF:1:100~1:200
Notes:Optimal dilutions/concentrations should be determined by the researcher.
Species Reactivity Human
Target Information
Alternative Names ML8
KU70
TLAA
CTC75
CTCBF
G22P1
Molecular Weight(MW) 69.8kDa
Database Links
Entrez Gene 2547
Application

Application

Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

Application

Figure 1: Western blot analysis using XRCC6 mAb against human XRCC6 (AA: 6-214) recombinant protein. (Expected MW is 49.7 kDa)

Application

Figure 2: Western blot analysis using XRCC6 mAb against HEK293 (1) and XRCC6 (AA: 6-214)-hIgGFc transfected HEK293 (2) cell lysate.

Application

Figure 3: Western blot analysis using XRCC6 mouse mAb against Hela (1), PC-2 (2), A549 (3), A431 (4), HepG2 (5), K562 (6) cell lysate.

Application

Figure 4: Immunofluorescence analysis of MCF-7 cells using XRCC6 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Application

Figure 5: Flow cytometric analysis of A431 cells using XRCC6 mouse mAb (green) and negative control (red).

Application

Figure 6: Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues using XRCC6 mouse mAb with DAB staining.

Application

Figure 7: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using XRCC6 mouse mAb with DAB staining.
Application Notes WB:1:250~1:1000
IHC:1:20~1:100
ICC:1:100~1:200
ICC/IF:1:100~1:200
Notes:Optimal dilutions/concentrations should be determined by the researcher.
Additional Information
Form Purified antibody in PBS with 0.05% sodium azide.
Storage Instructions 4℃; -20℃ for long term storage
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