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Black line: Control Antigen (100 ng);
Purple line: Antigen(10ng);
Blue line: Antigen (50 ng);
Red line: Antigen (100 ng);
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Figure 1: Western blot analysis using CSPG4 mAb against human CSPG4 recombinant protein. (Expected MW is 32.5 kDa)
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Figure 2: Western blot analysis using CSPG4 mAb against HEK293 (1) and CSPG4 (AA: 2247-2308)-hIgGFc transfected HEK293 (2) cell lysate.
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Figure 3: Flow cytometric analysis of HEK293 cells using CSPG4 mouse mAb (green) and negative control (red).