Application
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);Application
Figure 1: Western blot analysis using Ring1 mAb against human Ring1 recombinant protein. (Expected MW is 44.6 kDa)Application
Figure 2: Western blot analysis using Ring1 mAb against HEK293 (1) and Ring1 (AA: 79-263)-hIgGFc transfected HEK293 (2) cell lysate.Application
Figure 3: Western blot analysis using Ring1 mouse mAb against MOLT-4 (1), LNCaP (2), Hela (3), HEK-293 (4) and Jurkat (5) cell lysate.Application
Figure 4: Flow cytometric analysis of Hela cells using Ring1 mouse mAb (green) and negative control (red).Application
Figure 5: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using Ring1 mouse mAb with DAB staining.Application
Figure 6: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using Ring1 mouse mAb with DAB staining.| Product Name | Ring1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RNF1; RING1A |
| Immunogen | Purified recombinant fragment of human Ring1 (AA: 79-263) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Unknow |
| Tested Applications | WBIHCFCMICC/IF |
| WB:1:250~1:1000 IHC:1:20~1:100 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Alternative Names | RNF1 RING1A |
|---|---|
| Molecular Weight(MW) | 42.4kDa |
| Entrez Gene | 6015 |
|---|
Application
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Application
Figure 1: Western blot analysis using Ring1 mAb against human Ring1 recombinant protein. (Expected MW is 44.6 kDa)
Application
Figure 2: Western blot analysis using Ring1 mAb against HEK293 (1) and Ring1 (AA: 79-263)-hIgGFc transfected HEK293 (2) cell lysate.
Application
Figure 3: Western blot analysis using Ring1 mouse mAb against MOLT-4 (1), LNCaP (2), Hela (3), HEK-293 (4) and Jurkat (5) cell lysate.
Application
Figure 4: Flow cytometric analysis of Hela cells using Ring1 mouse mAb (green) and negative control (red).
Application
Figure 5: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using Ring1 mouse mAb with DAB staining.
Application
Figure 6: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using Ring1 mouse mAb with DAB staining.| Application Notes | WB:1:250~1:1000 IHC:1:20~1:100 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Purified antibody in PBS with 0.05% sodium azide |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
