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Black line: Control Antigen (100 ng);
Purple line: Antigen(10ng);
Blue line: Antigen (50 ng);
Red line: Antigen (100 ng);
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Figure 1: Western blot analysis using ABCG5 mAb against human ABCG5 recombinant protein. (Expected MW is 32.7 kDa)
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Figure 2: Western blot analysis using ABCG5 mAb against HEK293 (1) and ABCG5 (AA: 306-367)-hIgGFc transfected HEK293 (2) cell lysate.
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Figure 3: Western blot analysis using ABCG5 mouse mAb against HepG2 (1), HeLa (2), Jurkat (3), and Lovo (4) cell lysate.
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Figure 5: Flow cytometric analysis of HepG2 cells using ABCG5 mouse mAb (green) and negative control (purple).