Application
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);Application
Figure 1: Western blot analysis using CD33 mAb against human CD33 recombinant protein. (Expected MW is 49.2 kDa)Application
Figure 2: Western blot analysis using CD33 mAb against HEK293 (1) and CD33 (AA: 15-237)-hIgGFc transfected HEK293 (2) cell lysate.Application
Figure 5: Flow cytometric analysis of HepG2 cells using CD33 mouse mAb (green) and negative control (purple).Application
Figure 6: Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using CD33 mouse mAb with DAB staining.Application
Figure 7: Immunohistochemical analysis of paraffin-embedded endometrium tissues using CD33 mouse mAb with DAB staining.| Product Name | CD33 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | p67; SIGLEC3; SIGLEC-3 |
| Immunogen | Purified recombinant fragment of human CD33 (AA: 15-237) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Unknow |
| Tested Applications | WBIHCFCMICC/IF |
| WB:1:250~1:1000 IHC:1:20~1:100 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Alternative Names | p67 SIGLEC3 SIGLEC-3 |
|---|---|
| Molecular Weight(MW) | 39.8kDa |
| Entrez Gene | 945 |
|---|
Application
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Application
Figure 1: Western blot analysis using CD33 mAb against human CD33 recombinant protein. (Expected MW is 49.2 kDa)
Application
Figure 2: Western blot analysis using CD33 mAb against HEK293 (1) and CD33 (AA: 15-237)-hIgGFc transfected HEK293 (2) cell lysate.
Application
Figure 5: Flow cytometric analysis of HepG2 cells using CD33 mouse mAb (green) and negative control (purple).
Application
Figure 6: Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using CD33 mouse mAb with DAB staining.
Application
Figure 7: Immunohistochemical analysis of paraffin-embedded endometrium tissues using CD33 mouse mAb with DAB staining.| Application Notes | WB:1:250~1:1000 IHC:1:20~1:100 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Purified antibody in PBS with 0.05% sodium azide |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
