Application
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);Application
Figure 1: Western blot analysis using CLGN mAb against human CLGN recombinant protein. (Expected MW is 43.5 kDa)Application
Figure 2: Western blot analysis using CLGN mAb against HEK293 (1) and CLGN (AA: 249-405)-hIgGFc transfected HEK293 (2) cell lysate.Application
Figure 3: Western blot analysis using CLGN mouse mAb against LNCaP (1), HepG2 (2), PC-3 (3), and Raji (4) cell lysate.Application
Figure 4: Immunofluorescence analysis of HepG2 cells using CLGN mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.Application
Figure 5: Flow cytometric analysis of HEK293 cells using CLGN mouse mAb (green) and negative control (purple).Application
Figure 6: Immunohistochemical analysis of paraffin-embedded breast cancer tissues using CLGN mouse mAb with DAB staining.Application
Figure 7: Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using CLGN mouse mAb with DAB staining.| Product Name | CLGN |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Purified recombinant fragment of human CLGN (AA: 249-405) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Unknow |
| Tested Applications | WBIHCICCFCMICC/IF |
| WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Molecular Weight(MW) | 70kDa |
|---|
| Entrez Gene | 1047 |
|---|
Application
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Application
Figure 1: Western blot analysis using CLGN mAb against human CLGN recombinant protein. (Expected MW is 43.5 kDa)
Application
Figure 2: Western blot analysis using CLGN mAb against HEK293 (1) and CLGN (AA: 249-405)-hIgGFc transfected HEK293 (2) cell lysate.
Application
Figure 3: Western blot analysis using CLGN mouse mAb against LNCaP (1), HepG2 (2), PC-3 (3), and Raji (4) cell lysate.
Application
Figure 4: Immunofluorescence analysis of HepG2 cells using CLGN mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Application
Figure 5: Flow cytometric analysis of HEK293 cells using CLGN mouse mAb (green) and negative control (purple).
Application
Figure 6: Immunohistochemical analysis of paraffin-embedded breast cancer tissues using CLGN mouse mAb with DAB staining.
Application
Figure 7: Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using CLGN mouse mAb with DAB staining.| Application Notes | WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Purified antibody in PBS with 0.05% sodium azide |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
