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Black line: Control Antigen (100 ng);
Purple line: Antigen(10ng);
Blue line: Antigen (50 ng);
Red line: Antigen (100 ng);
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Figure 1: Western blot analysis using PDE1B mAb against human PDE1B recombinant protein. (Expected MW is 44.4 kDa)
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Figure 2: Western blot analysis using PDE1B mAb against HEK293 (1) and PDE1B (AA: 370-536)-hIgGFc transfected HEK293 (2) cell lysate.
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Figure 3: Western blot analysis using PDE1B mouse mAb against A549 (1), SK-MES-1 (2), PC-12 (4),3T3L1 (5) cell lysate and Mouse brain (3) tissue lysate.
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Figure 4: Flow cytometric analysis of A549 cells using PDE1B mouse mAb (green) and negative control (red).