Application
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);Application
Figure 1: Western blot analysis using WHSC2 mAb against human WHSC2 recombinant protein. (Expected MW is 50.2 kDa)Application
Figure 2: Western blot analysis using WHSC2 mAb against HEK293 (1) and WHSC2 (AA: 280-511)-hIgGFc transfected HEK293 (2) cell lysate.Application
Figure 3: Western blot analysis using WHSC2 mouse mAb against Jurkat (1), HeLa (2), HEK293 (3), A549 (4), and SPC-A-1 (5) cell lysate.Application
Figure 4: Immunofluorescence analysis of HeLa cells using WHSC2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.Application
Figure 5: Flow cytometric analysis of HeLa cells using WHSC2 mouse mAb (green) and negative control (purple).Application
Figure 6: Immunohistochemical analysis of paraffin-embedded colon cancer tissues using WHSC2 mouse mAb with DAB staining.Application
Figure 7: Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using WHSC2 mouse mAb with DAB staining.| Product Name | WHSC2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NELFA; NELF-A; P/OKcl.15 |
| Immunogen | Purified recombinant fragment of human WHSC2 (AA: 280-511) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Unknow |
| Tested Applications | WBIHCICCFCMICC/IF |
| WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Alternative Names | NELFA NELF-A P/OKcl.15 |
|---|---|
| Molecular Weight(MW) | 57.3kDa |
| Entrez Gene | 7469 |
|---|
Application
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Application
Figure 1: Western blot analysis using WHSC2 mAb against human WHSC2 recombinant protein. (Expected MW is 50.2 kDa)
Application
Figure 2: Western blot analysis using WHSC2 mAb against HEK293 (1) and WHSC2 (AA: 280-511)-hIgGFc transfected HEK293 (2) cell lysate.
Application
Figure 3: Western blot analysis using WHSC2 mouse mAb against Jurkat (1), HeLa (2), HEK293 (3), A549 (4), and SPC-A-1 (5) cell lysate.
Application
Figure 4: Immunofluorescence analysis of HeLa cells using WHSC2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Application
Figure 5: Flow cytometric analysis of HeLa cells using WHSC2 mouse mAb (green) and negative control (purple).
Application
Figure 6: Immunohistochemical analysis of paraffin-embedded colon cancer tissues using WHSC2 mouse mAb with DAB staining.
Application
Figure 7: Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using WHSC2 mouse mAb with DAB staining.| Application Notes | WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Ascitic fluid containing 0.03% sodium azide. |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
