Application
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);Application
Figure 1: Western blot analysis using SLC2A4 mAb against human SLC2A4 recombinant protein. (Expected MW is 39.9 kDa)Application
Figure 2: Western blot analysis using SLC2A4 mAb against HEK293 (1) and SLC2A4 (AA: 224-353)-hIgGFc transfected HEK293 (2) cell lysate.Application
Figure 3: Western blot analysis using SLC2A4 mouse mAb against NIH3T3 (1), 3T3L1 (2), MCF-7 (4) cell lysate and Mouse heart (3) tissue lysate.Application
Figure 4: Immunofluorescence analysis of HeLa cells using SLC2A4 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Application
Figure 5: Immunofluorescence analysis of HepG2 cells using SLC2A4 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Application
Figure 6: Flow cytometric analysis of HeLa cells using SLC2A4 mouse mAb (green) and negative control (purple).Application
Figure 7: Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using SLC2A4 mouse mAb with DAB staining.Application
Figure 8: Immunohistochemical analysis of paraffin-embedded cardiac muscle tissues using SLC2A4 mouse mAb with DAB staining.| Product Name | SLC2A4 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GLUT4 |
| Immunogen | Purified recombinant fragment of human SLC2A4 (AA: 224-353 ) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Unknow |
| Tested Applications | WBIHCICCFCMICC/IF |
| WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouse |
| Alternative Names | GLUT4 |
|---|---|
| Molecular Weight(MW) | 54.8kDa |
| Entrez Gene | 6517 |
|---|
Application
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Application
Figure 1: Western blot analysis using SLC2A4 mAb against human SLC2A4 recombinant protein. (Expected MW is 39.9 kDa)
Application
Figure 2: Western blot analysis using SLC2A4 mAb against HEK293 (1) and SLC2A4 (AA: 224-353)-hIgGFc transfected HEK293 (2) cell lysate.
Application
Figure 3: Western blot analysis using SLC2A4 mouse mAb against NIH3T3 (1), 3T3L1 (2), MCF-7 (4) cell lysate and Mouse heart (3) tissue lysate.
Application
Figure 4: Immunofluorescence analysis of HeLa cells using SLC2A4 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Application
Figure 5: Immunofluorescence analysis of HepG2 cells using SLC2A4 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Application
Figure 6: Flow cytometric analysis of HeLa cells using SLC2A4 mouse mAb (green) and negative control (purple).
Application
Figure 7: Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using SLC2A4 mouse mAb with DAB staining.
Application
Figure 8: Immunohistochemical analysis of paraffin-embedded cardiac muscle tissues using SLC2A4 mouse mAb with DAB staining.| Application Notes | WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Ascitic fluid containing 0.03% sodium azide. |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
