Application
Figure 1: Western blot analysis using GSK3B mouse mAb against A549 (1), K562 (2), PC-12 (3), NIH/3T3 (4), and HEK293 (5) cell lysate.Application
Figure 2: Immunohistochemical analysis of paraffin-embedded human lung cancer (left) and breast cancer tissues (right) using GSK3B mouse mAb with DAB staining.Application
Figure 3: Immunofluorescence analysis of NIH/3T3 (left) and U251 (right) cells using GSK3B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Application
Figure 4: Flow cytometric analysis of Hela cells using GSK3B mouse mAb (green) and negative control (purple).| Product Name | GSK3B |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GSK-3β; GSK3-beta |
| Immunogen | Purified recombinant fragment of human GSK3B expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Unknow |
| Tested Applications | WBIHCICCFCMICC/IF |
| WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseMonkeyRat |
| Alternative Names | GSK-3β GSK3-beta |
|---|---|
| Molecular Weight(MW) | 46kDa |
| Entrez Gene | 2932 |
|---|
Application
Figure 1: Western blot analysis using GSK3B mouse mAb against A549 (1), K562 (2), PC-12 (3), NIH/3T3 (4), and HEK293 (5) cell lysate.
Application
Figure 2: Immunohistochemical analysis of paraffin-embedded human lung cancer (left) and breast cancer tissues (right) using GSK3B mouse mAb with DAB staining.
Application
Figure 3: Immunofluorescence analysis of NIH/3T3 (left) and U251 (right) cells using GSK3B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Application
Figure 4: Flow cytometric analysis of Hela cells using GSK3B mouse mAb (green) and negative control (purple).| Application Notes | WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Ascitic fluid containing 0.03% sodium azide. |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
