Application
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);Application
Figure 1: Western blot analysis using ACLY mAb against human ACLY recombinant protein. (Expected MW is 46.7 kDa)Application
Figure 2: Western blot analysis using ACLY mouse mAb against HeLa (1), NIH3T3 (2), C6 (3), COS7 (4), and Raji (5) cell lysate.Application
Figure 3: Immunofluorescence analysis of HeLa cells using ACLY mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Application
Figure 4: Flow cytometric analysis of HeLa cells using ACLY mouse mAb (green) and negative control (purple).Application
Figure 5: Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using ACLY mouse mAb with DAB staining.Application
Figure 6: Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues using ACLY mouse mAb with DAB staining.| Product Name | ACLY |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ACL; ATPCL; CLATP |
| Immunogen | Purified recombinant fragment of human ACLY (AA: 306-502 ) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Unknow |
| Tested Applications | WBIHCICCFCMICC/IF |
| WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseMonkeyRat |
| Alternative Names | ACL ATPCL CLATP |
|---|---|
| Molecular Weight(MW) | 125kDa |
| Entrez Gene | 47 |
|---|
Application
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Application
Figure 1: Western blot analysis using ACLY mAb against human ACLY recombinant protein. (Expected MW is 46.7 kDa)
Application
Figure 2: Western blot analysis using ACLY mouse mAb against HeLa (1), NIH3T3 (2), C6 (3), COS7 (4), and Raji (5) cell lysate.
Application
Figure 3: Immunofluorescence analysis of HeLa cells using ACLY mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Application
Figure 4: Flow cytometric analysis of HeLa cells using ACLY mouse mAb (green) and negative control (purple).
Application
Figure 5: Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using ACLY mouse mAb with DAB staining.
Application
Figure 6: Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues using ACLY mouse mAb with DAB staining.| Application Notes | WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Purified antibody in PBS with 0.05% sodium azide |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
