Application
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);Application
Figure 1: Western blot analysis using ENO2 mAb against human ENO2 recombinant protein. (Expected MW is 46 kDa)Application
Figure 2: Western blot analysis using ENO2 mouse mAb against Mouse brain (1), NIH3T3 (2), and C6 (3) cell lysate.Application
Figure 3: Flow cytometric analysis of HeLa cells using ENO2 mouse mAb (green) and negative control (red).Application
Figure 4: Immunohistochemical analysis of paraffin-embedded lung cancer tissues using ENO2 mouse mAb with DAB staining.Application
Figure 5: Immunohistochemical analysis of paraffin-embedded liver cancer tissues using ENO2 mouse mAb with DAB staining.| Product Name | ENO2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NSE |
| Immunogen | Purified recombinant fragment of human ENO2 (AA: 251-433) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Unknow |
| Tested Applications | WBIHCFCMICC/IF |
| WB:1:250~1:1000 IHC:1:20~1:100 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouse |
| Alternative Names | NSE |
|---|---|
| Molecular Weight(MW) | 47.3kDa |
| Entrez Gene | 2026 |
|---|
Application
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Application
Figure 1: Western blot analysis using ENO2 mAb against human ENO2 recombinant protein. (Expected MW is 46 kDa)
Application
Figure 2: Western blot analysis using ENO2 mouse mAb against Mouse brain (1), NIH3T3 (2), and C6 (3) cell lysate.
Application
Figure 3: Flow cytometric analysis of HeLa cells using ENO2 mouse mAb (green) and negative control (red).
Application
Figure 4: Immunohistochemical analysis of paraffin-embedded lung cancer tissues using ENO2 mouse mAb with DAB staining.
Application
Figure 5: Immunohistochemical analysis of paraffin-embedded liver cancer tissues using ENO2 mouse mAb with DAB staining.| Application Notes | WB:1:250~1:1000 IHC:1:20~1:100 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Purified antibody in PBS with 0.05% sodium azide |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
