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Black line: Control Antigen (100 ng);
Purple line: Antigen(10ng);
Blue line: Antigen (50 ng);
Red line: Antigen (100 ng);
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Figure 1: Western blot analysis using CD5 mAb against human CD5 recombinant protein. (Expected MW is 48.3 kDa)
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Figure 2: Western blot analysis using CD5 mouse mAb against K562 (1), Jurkat (2), Raji (3), and MOLT4 (4) cell lysate.
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Figure 3: Flow cytometric analysis of Jurkat cells using CD5 mouse mAb (green) and negative control (red).