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E2F1

Catalog Number:OM640811
Human;Mouse
WB;IHC;FCM;ICC/IF
OM640811
Discount Price
OM640811-1.0mg/ml
1~2 Week
1.0mg/ml
1~2 Week
Product Profile
Product Name E2F1
Antibody Type Primary Antibodies
Antigen Alias RBP3; E2F-1; RBAP1; RBBP3
Immunogen Purified recombinant fragment of human E2F1 (AA: 69-223) expressed in E. Coli.
Key Feature
Clonality Monoclonal
Host Species Unknow
Tested Applications WBIHCFCMICC/IF
WB:1:250~1:1000
IHC:1:20~1:100
ICC/IF:1:100~1:200
Notes:Optimal dilutions/concentrations should be determined by the researcher.
Species Reactivity HumanMouse
Target Information
Alternative Names RBP3
E2F-1
RBAP1
RBBP3
Molecular Weight(MW) 46.9kDa
Database Links
Entrez Gene 1869
Application

Application

Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

Application

Figure 1: Western blot analysis using E2F1 mAb against human E2F1 recombinant protein. (Expected MW is 42.7 kDa)

Application

Figure 2: Western blot analysis using E2F1 mouse mAb against HeLa (1), SK-N-SH (2), and NIH3T3 (3) cell lysate.

Application

Figure 3: Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using E2F1 mouse mAb with DAB staining.

Application

Figure 4: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using E2F1 mouse mAb with DAB staining.

Application

Figure 5: Flow cytometric analysis of HeLa cells using E2F1 mouse mAb (green) and negative control (red).
Application Notes WB:1:250~1:1000
IHC:1:20~1:100
ICC/IF:1:100~1:200
Notes:Optimal dilutions/concentrations should be determined by the researcher.
Additional Information
Form Purified antibody in PBS with 0.05% sodium azide
Storage Instructions 4℃; -20℃ for long term storage
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