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Black line: Control Antigen (100 ng);
Purple line: Antigen(10ng);
Blue line: Antigen (50 ng);
Red line: Antigen (100 ng);
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Figure 1: Western blot analysis using PPM1A mAb against human PPM1A recombinant protein. (Expected MW is 45.9 kDa)
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Figure 2: Western blot analysis using PPM1A mAb against HEK293 (1) and PPM1A (AA: 202-382)-hIgGFc transfected HEK293 (2) cell lysate.
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Figure 3: Western blot analysis using PPM1A mouse mAb against Jurkat (1), Jurkat (2), A431 (3), HeLa (4), HEK293 (5), Raji (6), MCF-7 (7), and COS7 (8) cell lysate.
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Figure 4: Flow cytometric analysis of HeLa cells using PPM1A mouse mAb (green) and negative control (red).