Application
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);Application
Figure 1: Western blot analysis using SHH mAb against human SHH recombinant protein. (Expected MW is 41 kDa)Application
Figure 2: Western blot analysis using SHH mAb against HEK293 (1) and SHH (AA: 26-161)-hIgGFc transfected HEK293 (2) cell lysate.Application
Figure 3: Western blot analysis using SHH mouse mAb against LNCaP (1), HepG2 (2), PANC-1 (3),HeLa (4), SK-N-SH (5), F9 (6), NIH3T3 (7), and COS7 (8) cell lysate.Application
Figure 4: Immunohistochemical analysis of paraffin-embedded liver cancer tissues using SHH mouse mAb with DAB staining.Application
Figure 5: Flow cytometric analysis of HeLa cells using SHH mouse mAb (green) and negative control (red).| Product Name | SHH |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TPT; HHG1; HLP3; HPE3; SMMCI; TPTPS; MCOPCB5 |
| Immunogen | Purified recombinant fragment of human SHH (AA: 26-161) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Unknow |
| Tested Applications | WBIHCFCMICC/IF |
| WB:1:250~1:1000 IHC:1:20~1:100 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseMonkey |
| Alternative Names | TPT HHG1 HLP3 HPE3 SMMCI TPTPS MCOPCB5 |
|---|---|
| Molecular Weight(MW) | 49.6kDa |
| Entrez Gene | 6469 |
|---|
Application
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Application
Figure 1: Western blot analysis using SHH mAb against human SHH recombinant protein. (Expected MW is 41 kDa)
Application
Figure 2: Western blot analysis using SHH mAb against HEK293 (1) and SHH (AA: 26-161)-hIgGFc transfected HEK293 (2) cell lysate.
Application
Figure 3: Western blot analysis using SHH mouse mAb against LNCaP (1), HepG2 (2), PANC-1 (3),HeLa (4), SK-N-SH (5), F9 (6), NIH3T3 (7), and COS7 (8) cell lysate.
Application
Figure 4: Immunohistochemical analysis of paraffin-embedded liver cancer tissues using SHH mouse mAb with DAB staining.
Application
Figure 5: Flow cytometric analysis of HeLa cells using SHH mouse mAb (green) and negative control (red).| Application Notes | WB:1:250~1:1000 IHC:1:20~1:100 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Purified antibody in PBS with 0.05% sodium azide |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
