Application
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);Application
Figure 1: Western blot analysis using GPC3 mAb against human GPC3 (AA: 55-200) recombinant protein. (Expected MW is 28.5 kDa)Application
Figure 2: Western blot analysis using GPC3 mouse mAb against HepG2 (1), HEK293 (2), Jurkat (3), SK-N-SH (4), PC-12 (5), F9 (6)and Mouse liver (7) cell lysate.Application
Figure 3: Immunohistochemical analysis of paraffin-embedded liver cancer tissues using GPC3 mouse mAb with DAB staining.Application
Figure 4: Immunohistochemical analysis of paraffin-embedded breast cancer tissues using GPC3 mouse mAb with DAB staining.Application
Figure 5: Immunofluorescence analysis of HeLa cells using GPC3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Application
Figure 6: Flow cytometric analysis of Jurkat cells using GPC3 mouse mAb (green) and negative control (red).| Product Name | GPC3 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SGB; DGSX; MXR7; SDYS; SGBS; OCI-5; SGBS1; GTR2-2 |
| Immunogen | Purified recombinant fragment of human GPC3 expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Unknow |
| Tested Applications | WBIHCICCFCMICC/IF |
| WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Alternative Names | SGB DGSX MXR7 SDYS SGBS OCI-5 SGBS1 GTR2-2 |
|---|---|
| Molecular Weight(MW) | 65.5kDa |
| Entrez Gene | 2719 |
|---|
Application
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Application
Figure 1: Western blot analysis using GPC3 mAb against human GPC3 (AA: 55-200) recombinant protein. (Expected MW is 28.5 kDa)
Application
Figure 2: Western blot analysis using GPC3 mouse mAb against HepG2 (1), HEK293 (2), Jurkat (3), SK-N-SH (4), PC-12 (5), F9 (6)and Mouse liver (7) cell lysate.
Application
Figure 3: Immunohistochemical analysis of paraffin-embedded liver cancer tissues using GPC3 mouse mAb with DAB staining.
Application
Figure 4: Immunohistochemical analysis of paraffin-embedded breast cancer tissues using GPC3 mouse mAb with DAB staining.
Application
Figure 5: Immunofluorescence analysis of HeLa cells using GPC3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Application
Figure 6: Flow cytometric analysis of Jurkat cells using GPC3 mouse mAb (green) and negative control (red).| Application Notes | WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Purified antibody in PBS with 0.05% sodium azide |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
