Application
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);Application
Figure 1: Western blot analysis using NOS2 mAb against human NOS2 (AA: 997-1058) recombinant protein. (Expected MW is 32.6 kDa)Application
Figure 2: Western blot analysis using NOS2 mouse mAb against Jurkat (1), Jurkat (2), A549 (3), HeLa (4), NIH3T3 (5)and MCF-7 (6) cell lysate.Application
Figure 3: Immunohistochemical analysis of paraffin-embedded liver cancer tissues using NOS2 mouse mAb with DAB staining.Application
Figure 4: Immunohistochemical analysis of paraffin-embedded breast cancer tissues using NOS2 mouse mAb with DAB staining.Application
Figure 5: Flow cytometric analysis of MCF-7 cells using NOS2 mouse mAb (green) and negative control (red).| Product Name | NOS2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NOS; INOS; NOS2A; HEP-NOS |
| Immunogen | Purified recombinant fragment of human NOS2 expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Unknow |
| Tested Applications | WBIHCFCMICC/IF |
| WB:1:250~1:1000 IHC:1:20~1:100 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouse |
| Alternative Names | NOS INOS NOS2A HEP-NOS |
|---|---|
| Molecular Weight(MW) | 131kDa |
| Entrez Gene | 4843 |
|---|
Application
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Application
Figure 1: Western blot analysis using NOS2 mAb against human NOS2 (AA: 997-1058) recombinant protein. (Expected MW is 32.6 kDa)
Application
Figure 2: Western blot analysis using NOS2 mouse mAb against Jurkat (1), Jurkat (2), A549 (3), HeLa (4), NIH3T3 (5)and MCF-7 (6) cell lysate.
Application
Figure 3: Immunohistochemical analysis of paraffin-embedded liver cancer tissues using NOS2 mouse mAb with DAB staining.
Application
Figure 4: Immunohistochemical analysis of paraffin-embedded breast cancer tissues using NOS2 mouse mAb with DAB staining.
Application
Figure 5: Flow cytometric analysis of MCF-7 cells using NOS2 mouse mAb (green) and negative control (red).| Application Notes | WB:1:250~1:1000 IHC:1:20~1:100 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Purified antibody in PBS with 0.05% sodium azide |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
