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PBK

Catalog Number:OM640854
Human
WB;IHC;ICC;FCM;ICC/IF
OM640854
Discount Price
OM640854-100μl
1~2 Week
100μl
1~2 Week
Product Profile
Product Name PBK
Antibody Type Primary Antibodies
Antigen Alias SPK; CT84; TOPK; Nori-3
Immunogen Purified recombinant fragment of human PBK expressed in E. Coli.
Key Feature
Clonality Monoclonal
Host Species Unknow
Tested Applications WBIHCICCFCMICC/IF
WB:1:250~1:1000
IHC:1:20~1:100
ICC:1:100~1:200
ICC/IF:1:100~1:200
Notes:Optimal dilutions/concentrations should be determined by the researcher.
Species Reactivity Human
Target Information
Alternative Names SPK
CT84
TOPK
Nori-3
Molecular Weight(MW) 36kDa
Database Links
Entrez Gene 55872
Application

Application

Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

Application

Figure5: Immunohistochemical analysis of paraffin-embedded colon cancer tissues using PBK mouse mAb with DAB staining.

Application

Figure 2: Western blot analysis using PBK mAb against human PBK (AA: 50-230) recombinant protein. (Expected MW is 45.8 kDa)

Application

Figure 3: Western blot analysis using PBK mouse mAb against A431 (1) cell lysate.

Application

Figure 4: Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using PBK mouse mAb with DAB staining.

Application

Figure 6: Immunofluorescence analysis of Hela cells using PBK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Application

Figure 7: Flow cytometric analysis of Hela cells using PBK mouse mAb (green) and negative control (red).
Application Notes WB:1:250~1:1000
IHC:1:20~1:100
ICC:1:100~1:200
ICC/IF:1:100~1:200
Notes:Optimal dilutions/concentrations should be determined by the researcher.
Additional Information
Form Ascitic fluid containing 0.03% sodium azide.
Storage Instructions 4℃; -20℃ for long term storage
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