Application
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);Application
Figure 1: Western blot analysis using PIK3CA mAb against human PIK3CA (AA: 881-1068) recombinant protein. (Expected MW is 47.4 kDa)Application
Figure 2: Western blot analysis using PIK3CA mAb against HEK293 (1) and PIK3CA (AA: 881-1068)-hIgGFc transfected HEK293 (2) cell lysate.Application
Figure 3: Immunofluorescence analysis of HeLa cells using PIK3CA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.Application
Figure 4: Flow cytometric analysis of Jurkat cells using PIK3CA mouse mAb (green) and negative control (red).| Product Name | PIK3CA |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PI3K; p110-alpha |
| Immunogen | Purified recombinant fragment of human PIK3CA expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Unknow |
| Tested Applications | WBICCFCMICC/IF |
| WB:1:250~1:1000 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Alternative Names | PI3K p110-alpha |
|---|---|
| Molecular Weight(MW) | 124kDa |
| Entrez Gene | 5290 |
|---|
Application
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Application
Figure 1: Western blot analysis using PIK3CA mAb against human PIK3CA (AA: 881-1068) recombinant protein. (Expected MW is 47.4 kDa)
Application
Figure 2: Western blot analysis using PIK3CA mAb against HEK293 (1) and PIK3CA (AA: 881-1068)-hIgGFc transfected HEK293 (2) cell lysate.
Application
Figure 3: Immunofluorescence analysis of HeLa cells using PIK3CA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Application
Figure 4: Flow cytometric analysis of Jurkat cells using PIK3CA mouse mAb (green) and negative control (red).| Application Notes | WB:1:250~1:1000 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Purified antibody in PBS with 0.05% sodium azide |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
