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Black line: Control Antigen (100 ng);
Purple line: Antigen(10ng);
Blue line: Antigen (50 ng);
Red line: Antigen (100 ng);
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Figure 1: Western blot analysis using SOX9 mAb against human SOX9 (AA: 147-306) recombinant protein. (Expected MW is 56 kDa)
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Figure 2: Western blot analysis using SOX9 mouse mAb against Lovo (1) and SW620 (2) cell lysate.
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Figure 3: Immunofluorescence analysis of Hela cells using SOX9 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.