Application
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);Application
Figure 1: Western blot analysis using NEFL mAb against human NEFL (AA: 422-543) recombinant protein. (Expected MW is 62 kDa)Application
Figure 2: Western blot analysis using NEFL mouse mAb against Hela (1) and Jurkat (2) cell lysate.Application
Figure 3: Immunofluorescence analysis of Hela cells using NEFL mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Application
Figure 3: Immunohistochemical analysis of paraffin-embedded lung cancer tissues using NEFL mouse mAb with DAB staining.Application
Figure 4: Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using NEFL mouse mAb with DAB staining.Application
Figure 4: Flow cytometric analysis of Jurkat cells using NEFL mouse mAb (green) and negative control (red).| Product Name | NEFL |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NFL; NF-L; NF68; CMT1F; CMT2E |
| Immunogen | Purified recombinant fragment of human NEFL expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Unknow |
| Tested Applications | WBIHCICCFCMICC/IF |
| WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Alternative Names | NFL NF-L NF68 CMT1F CMT2E |
|---|---|
| Molecular Weight(MW) | 62kDa |
| Entrez Gene | 4747 |
|---|
Application
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Application
Figure 1: Western blot analysis using NEFL mAb against human NEFL (AA: 422-543) recombinant protein. (Expected MW is 62 kDa)
Application
Figure 2: Western blot analysis using NEFL mouse mAb against Hela (1) and Jurkat (2) cell lysate.
Application
Figure 3: Immunofluorescence analysis of Hela cells using NEFL mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Application
Figure 3: Immunohistochemical analysis of paraffin-embedded lung cancer tissues using NEFL mouse mAb with DAB staining.
Application
Figure 4: Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using NEFL mouse mAb with DAB staining.
Application
Figure 4: Flow cytometric analysis of Jurkat cells using NEFL mouse mAb (green) and negative control (red).| Application Notes | WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Ascitic fluid containing 0.03% sodium azide |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
