Application
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);Application
Figure 1: Western blot analysis using BIRC5 mAb against human BIRC5 (AA: 1-142) recombinant protein. (Expected MW is 42 kDa)Application
Figure 2: Western blot analysis using BIRC5 mAb against HEK293 (1) and BIRC5 (AA: 1-142)-hIgGFc transfected HEK293 (2) cell lysate.Application
Figure 3: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using BIRC5 mouse mAb with DAB staining.Application
Figure 4: Immunohistochemical analysis of paraffin-embedded colon cancer tissues using BIRC5 mouse mAb with DAB staining.Application
Figure 5: Immunofluorescence analysis of MSCS cells using BIRC5 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Application
Figure 6: Flow cytometric analysis of Jurkat cells using BIRC5 mouse mAb (green) and negative control (red).| Product Name | BIRC5 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | API4; EPR-1 |
| Immunogen | Purified recombinant fragment of human BIRC5 expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Unknow |
| Tested Applications | WBIHCICCFCMICC/IF |
| WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Alternative Names | API4 EPR-1 |
|---|---|
| Molecular Weight(MW) | 16kDa |
| Entrez Gene | 332 |
|---|
Application
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Application
Figure 1: Western blot analysis using BIRC5 mAb against human BIRC5 (AA: 1-142) recombinant protein. (Expected MW is 42 kDa)
Application
Figure 2: Western blot analysis using BIRC5 mAb against HEK293 (1) and BIRC5 (AA: 1-142)-hIgGFc transfected HEK293 (2) cell lysate.
Application
Figure 3: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using BIRC5 mouse mAb with DAB staining.
Application
Figure 4: Immunohistochemical analysis of paraffin-embedded colon cancer tissues using BIRC5 mouse mAb with DAB staining.
Application
Figure 5: Immunofluorescence analysis of MSCS cells using BIRC5 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Application
Figure 6: Flow cytometric analysis of Jurkat cells using BIRC5 mouse mAb (green) and negative control (red).| Application Notes | WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Purified antibody in PBS with 0.05% sodium azide |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
