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Black line: Control Antigen (100 ng);
Purple line: Antigen(10ng);
Blue line: Antigen (50 ng);
Red line: Antigen (100 ng);
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Figure 1: Western blot analysis using MSH6 mAb against human MSH6 (AA: 217-395) recombinant protein. (Expected MW is 45.5 kDa)
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Figure 2: Western blot analysis using MSH6 mouse mAb against HEK293 (1), HCT116 (2), A549 (3), A431 (4), MCF-7 (5) and HepG2 (6) cell lysate.
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Figure 3: Immunohistochemical analysis of paraffin-embedded colon cancer tissues using MSH6 mouse mAb with DAB staining.
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Figure 4: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using MSH6 mouse mAb with DAB staining.
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Figure 5: Immunofluorescence analysis of Hela cells using MSH6 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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Figure 6: Flow cytometric analysis of MCF-7 cells using MSH6 mouse mAb (green) and negative control (red).