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SKP1
Catalog Number:OM640902
Human
WB;IHC;ICC;FCM;ICC/IF
OM640902
Discount Price
OM640902-100μl
1~2 Week
100μl
1~2 Week
Product Profile
Product Name SKP1
Antibody Type Primary Antibodies
Antigen Alias OCP2; p19A; EMC19; SKP1A; OCP-II; TCEB1L; MGC34403
Immunogen Purified recombinant fragment of human SKP1 expressed in E. Coli.
Key Feature
Clonality Monoclonal
Host Species Unknow
Tested Applications WBIHCICCFCMICC/IF
WB:1:250~1:1000
IHC:1:20~1:100
ICC:1:100~1:200
ICC/IF:1:100~1:200
Notes:Optimal dilutions/concentrations should be determined by the researcher.
Species Reactivity Human
Target Information
Alternative Names OCP2
p19A
EMC19
SKP1A
OCP-II
TCEB1L
MGC34403
Molecular Weight(MW) 19kDa
Database Links
Entrez Gene 6500
Application

Application

Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

Application

Figure 1: Western blot analysis using SKP1 mAb against human SKP1 (AA: 1-160) recombinant protein. (Expected MW is 43.5 kDa)

Application

Figure 2: Western blot analysis using SKP1 mouse mAb against Hela (1), NIH/3T3 (2), A431 (3), RAJI (4), PC-12 (5), Cos7 (6), MCF-7 (7) and HepG2 (8) cell lysate.

Application

Figure 3: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using SKP1 mouse mAb with DAB staining.

Application

Figure 4: Immunofluorescence analysis of Hela cells using SKP1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Application

Figure 5: Flow cytometric analysis of NIH/3T3 cells using SKP1 mouse mAb (green) and negative control (red).
Application Notes WB:1:250~1:1000
IHC:1:20~1:100
ICC:1:100~1:200
ICC/IF:1:100~1:200
Notes:Optimal dilutions/concentrations should be determined by the researcher.
Additional Information
Form Ascitic fluid containing 0.03% sodium azide.
Storage Instructions 4℃; -20℃ for long term storage
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