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Black line: Control Antigen (100 ng);
Purple line: Antigen(10ng);
Blue line: Antigen (50 ng);
Red line: Antigen (100 ng);
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Figure 1: Western blot analysis using RSK2 mAb against human RSK2 (AA: 1-212) recombinant protein. (Expected MW is 49.7 kDa)
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Figure 2: Western blot analysis using RSK2 mouse mAb against Hela (1), MCF-7 (2), and HepG2 (3) cell lysate.
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Figure 3: Immunofluorescence analysis of HepG2 cells using RSK2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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Figure 4: Flow cytometric analysis of HepG2 cells using RSK2 mouse mAb (green) and negative control (red).