Application
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);Application
Figure 1: Western blot analysis using IL1B mAb against human IL1B (AA: 126-261) recombinant protein. (Expected MW is 41 kDa)Application
Figure 2: Western blot analysis using IL1B mAb against HEK293 (1) and IL1B-hIgGFc transfected HEK293 (2) cell lysate.Application
Figure 3: Immunohistochemical analysis of paraffin-embedded muscle tissues using IL1B mouse mAb with DAB staining.Application
Figure 4: Immunohistochemical analysis of paraffin-embedded lung cancer tissues using IL1B mouse mAb with DAB staining.Application
Figure 5: Immunofluorescence analysis of HepG2 cells using IL1B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.| Product Name | IL1B |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IL-1; IL1F2; IL1-BETA |
| Immunogen | Purified recombinant fragment of human IL1B expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Unknow |
| Tested Applications | WBIHCICCICC/IF |
| WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Alternative Names | IL-1 IL1F2 IL1-BETA |
|---|---|
| Molecular Weight(MW) | 31kDa |
| Entrez Gene | 3553 |
|---|
Application
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Application
Figure 1: Western blot analysis using IL1B mAb against human IL1B (AA: 126-261) recombinant protein. (Expected MW is 41 kDa)
Application
Figure 2: Western blot analysis using IL1B mAb against HEK293 (1) and IL1B-hIgGFc transfected HEK293 (2) cell lysate.
Application
Figure 3: Immunohistochemical analysis of paraffin-embedded muscle tissues using IL1B mouse mAb with DAB staining.
Application
Figure 4: Immunohistochemical analysis of paraffin-embedded lung cancer tissues using IL1B mouse mAb with DAB staining.
Application
Figure 5: Immunofluorescence analysis of HepG2 cells using IL1B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.| Application Notes | WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Ascitic fluid containing 0.03% sodium azide. |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
