Application
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);Application
Figure 1: Western blot analysis using MAPK3 mAb against human MAPK3 (AA: 9-143) recombinant protein. (Expected MW is 40.8 kDa)Application
Figure 2: Western blot analysis using MAPK3 mouse mAb against Hela (1), Jurkat (2), RAW264.7 (3), HEK293 (4), K562 (5), NIH/3T3 (6), Cos7 (7) and PC-12 (8) cell lysate.Application
Figure 3: Immunohistochemical analysis of paraffin-embedded breast cancer tissues using MAPK3 mouse mAb with DAB staining.Application
Figure 4: Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using MAPK3 mouse mAb with DAB staining.Application
Figure 5: Immunofluorescence analysis of NIH/3T3 cells using MAPK3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Application
Figure 6: Flow cytometric analysis of Hela cells using MAPK3 mouse mAb (blue) and negative control (red).| Product Name | MAPK3 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ERK1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; MGC20180 |
| Immunogen | Purified recombinant fragment of human MAPK3 expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Unknow |
| Tested Applications | WBIHCICCFCMICC/IF |
| WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRatMonkey |
| Alternative Names | ERK1 PRKM3 P44ERK1 P44MAPK HS44KDAP HUMKER1A MGC20180 |
|---|---|
| Molecular Weight(MW) | 44kDa |
| Entrez Gene | 5595 |
|---|
Application
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Application
Figure 1: Western blot analysis using MAPK3 mAb against human MAPK3 (AA: 9-143) recombinant protein. (Expected MW is 40.8 kDa)
Application
Figure 2: Western blot analysis using MAPK3 mouse mAb against Hela (1), Jurkat (2), RAW264.7 (3), HEK293 (4), K562 (5), NIH/3T3 (6), Cos7 (7) and PC-12 (8) cell lysate.
Application
Figure 3: Immunohistochemical analysis of paraffin-embedded breast cancer tissues using MAPK3 mouse mAb with DAB staining.
Application
Figure 4: Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using MAPK3 mouse mAb with DAB staining.
Application
Figure 5: Immunofluorescence analysis of NIH/3T3 cells using MAPK3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Application
Figure 6: Flow cytometric analysis of Hela cells using MAPK3 mouse mAb (blue) and negative control (red).| Application Notes | WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Ascitic fluid containing 0.03% sodium azide. |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
