Application
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);Application
Figure 1: Western blot analysis using CRK mAb against human CRK (AA: 1-204) recombinant protein. (Expected MW is 48.4 kDa)Application
Figure 2: Western blot analysis using CRK mAb against HEK293 (1) and CRK(AA: 1-204)-hIgGFc transfected HEK293 (2) cell lysate.Application
Figure 3: Immunohistochemical analysis of paraffin-embedded intima cancer tissues using CRK mouse mAb with DAB staining.Application
Figure 4: Immunohistochemical analysis of paraffin-embedded lung cancer tissues using CRK mouse mAb with DAB staining.Application
Figure 5: Immunofluorescence analysis of 3T3-L1 cells using CRK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Application
Figure 6: Flow cytometric analysis of Hela cells using CRK mouse mAb (blue) and negative control (red).| Product Name | CRK |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CRKII |
| Immunogen | Purified recombinant fragment of human CRK expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Unknow |
| Tested Applications | WBIHCICCFCMICC/IF |
| WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Alternative Names | CRKII |
|---|---|
| Molecular Weight(MW) | 42kDa |
| Entrez Gene | 1398 |
|---|
Application
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Application
Figure 1: Western blot analysis using CRK mAb against human CRK (AA: 1-204) recombinant protein. (Expected MW is 48.4 kDa)
Application
Figure 2: Western blot analysis using CRK mAb against HEK293 (1) and CRK(AA: 1-204)-hIgGFc transfected HEK293 (2) cell lysate.
Application
Figure 3: Immunohistochemical analysis of paraffin-embedded intima cancer tissues using CRK mouse mAb with DAB staining.
Application
Figure 4: Immunohistochemical analysis of paraffin-embedded lung cancer tissues using CRK mouse mAb with DAB staining.
Application
Figure 5: Immunofluorescence analysis of 3T3-L1 cells using CRK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Application
Figure 6: Flow cytometric analysis of Hela cells using CRK mouse mAb (blue) and negative control (red).| Application Notes | WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Ascitic fluid containing 0.03% sodium azide. |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
