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C-CBL

Catalog Number:OM640941
Human;Mouse;Rat
WB;IHC;ICC;FCM;ICC/IF
OM640941
Discount Price
OM640941-100μl
1~2 Week
100μl
1~2 Week
Product Profile
Product Name C-CBL
Antibody Type Primary Antibodies
Antigen Alias CBL; CBL2; NSLL; C-CBL; RNF55
Immunogen Purified recombinant fragment of human C-CBL expressed in E. Coli.
Key Feature
Clonality Monoclonal
Host Species Unknow
Tested Applications WBIHCICCFCMICC/IF
WB:1:250~1:1000
IHC:1:20~1:100
ICC:1:100~1:200
ICC/IF:1:100~1:200
Notes:Optimal dilutions/concentrations should be determined by the researcher.
Species Reactivity HumanMouseRat
Target Information
Alternative Names CBL
CBL2
NSLL
C-CBL
RNF55
Molecular Weight(MW) 120kDa
Database Links
Entrez Gene 867
Application

Application

Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Application

Figure 1: Western blot analysis using C-CBL mAb against human C-CBL (AA: 684-865) recombinant protein. (Expected MW is 44.9 kDa)

Application

Figure 2: Western blot analysis using C-CBL mouse mAb against RAJI (1), RAW264.7 (2), K562 (3), SKBR-3 (4), 3T3-L1 (5), THP-1 (6) and PC-12 (7) cell lysate.

Application

Figure 3: Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using C-CBL mouse mAb with DAB staining.

Application

Figure 4: Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using C-CBL mouse mAb with DAB staining.

Application

Figure 5: Immunofluorescence analysis of Hela cells using C-CBL mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Application

Figure 6: Flow cytometric analysis of MCF-7 cells using C-CBL mouse mAb (blue) and negative control (red).
Application Notes WB:1:250~1:1000
IHC:1:20~1:100
ICC:1:100~1:200
ICC/IF:1:100~1:200
Notes:Optimal dilutions/concentrations should be determined by the researcher.
Additional Information
Form Ascitic fluid containing 0.03% sodium azide.
Storage Instructions 4℃; -20℃ for long term storage
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