Application
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);Application
Figure 1: Western blot analysis using AIF mAb against human AIF (AA: 1-261) recombinant protein. (Expected MW is 35.6 kDa)Application
Figure 2: Western blot analysis using AIF mouse mAb against NIH/3T3 (1), Jurkat (2), Hela (3), HepG2 (4), MOLT4 (5), C6 (6), RAJI (7), Cos7 (8) and PC-12 (9) cell lysate.Application
Figure 3: Immunohistochemical analysis of paraffin-embedded human breast cancer tissues using AIF mouse mAb with DAB staining.Application
Figure 4: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using AIF mouse mAb with DAB staining.Application
Figure 5: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using AIF mouse mAb with DAB staining.Application
Figure 6: Immunohistochemical analysis of paraffin-embedded human lung cancer tissues using AIF mouse mAb with DAB staining.Application
Figure 7: Immunofluorescence analysis of NIH/3T3 cells using AIF mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Application
Figure 8: Flow cytometric analysis of HepG2 cells using AIF mouse mAb (blue) and negative control (red).| Product Name | AIF |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AIFM1; AIF; PDCD8; COXPD6; MGC111425 |
| Immunogen | Purified recombinant fragment of human AIF expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Unknow |
| Tested Applications | WBIHCICCFCMICC/IF |
| WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseMonkeyRat |
| Alternative Names | AIFM1 AIF PDCD8 COXPD6 MGC111425 |
|---|---|
| Molecular Weight(MW) | 67kDa |
| Entrez Gene | 9131 |
|---|
Application
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Application
Figure 1: Western blot analysis using AIF mAb against human AIF (AA: 1-261) recombinant protein. (Expected MW is 35.6 kDa)
Application
Figure 2: Western blot analysis using AIF mouse mAb against NIH/3T3 (1), Jurkat (2), Hela (3), HepG2 (4), MOLT4 (5), C6 (6), RAJI (7), Cos7 (8) and PC-12 (9) cell lysate.
Application
Figure 3: Immunohistochemical analysis of paraffin-embedded human breast cancer tissues using AIF mouse mAb with DAB staining.
Application
Figure 4: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using AIF mouse mAb with DAB staining.
Application
Figure 5: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using AIF mouse mAb with DAB staining.
Application
Figure 6: Immunohistochemical analysis of paraffin-embedded human lung cancer tissues using AIF mouse mAb with DAB staining.
Application
Figure 7: Immunofluorescence analysis of NIH/3T3 cells using AIF mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Application
Figure 8: Flow cytometric analysis of HepG2 cells using AIF mouse mAb (blue) and negative control (red).| Application Notes | WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Ascitic fluid containing 0.03% sodium azide. |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
