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Red: Control Antigen (100ng);
Purple: Antigen (10ng);
Green: Antigen (50ng);
Blue: Antigen (100ng);
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Figure 1: Western blot analysis using ITK mAb against human ITK (AA: 2-120) recombinant protein. (Expected MW is 39.7 kDa)
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Figure 2: Western blot analysis using ITK mouse mAb against Jurkat cell lysate.
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Figure 3: Immunofluorescence analysis of Jurkat cells using ITK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
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Figure 4: Flow cytometric analysis of Jurkat cells using ITK mouse mAb (blue) and negative control (red).