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NFKB1

Catalog Number:OM640973
Human
WB;IHC;ICC;FCM;ICC/IF
OM640973
Discount Price
OM640973-100μl
1~2 Week
100μl
1~2 Week
Product Profile
Product Name NFKB1
Antibody Type Primary Antibodies
Antigen Alias p50; KBF1; p105; EBP-1; MGC54151; NFKB-p50; NFkappaB; NF-kappaB; NFKB-p105; NF-kappa-B
Immunogen Purified recombinant fragment of human NFKB1 expressed in E. Coli.
Key Feature
Clonality Monoclonal
Host Species Unknow
Tested Applications WBIHCICCFCMICC/IF
WB:1:250~1:1000
IHC:1:20~1:100
ICC:1:100~1:200
ICC/IF:1:100~1:200
Notes:Optimal dilutions/concentrations should be determined by the researcher.
Species Reactivity Human
Target Information
Alternative Names p50
KBF1
p105
EBP-1
MGC54151
NFKB-p50
NFkappaB
NF-kappaB
NFKB-p105
NF-kappa-B
Molecular Weight(MW) 50kDa/105kDa
Database Links
Entrez Gene 4790
Application

Application

Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Application

Figure 1: Western blot analysis using NFKB1 mouse mAb against K562 (1), Jurkat (2), A431 (3), Hela (4), THP-1 (5) and MCF-7 (6) cell lysate.

Application

Figure 2: Immunohistochemical analysis of paraffin-embedded human intima cancer tissues (left) and human bladder cancer tissues (right) using NFKB1 mouse mAb with DAB staining.

Application

Figure 3: Immunofluorescence analysis of U251 cells using NFKB1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Application

Figure 3: Flow cytometric analysis of MCF-7 cells using NFKB1 mouse mAb (green) and negative control (purple).
Application Notes WB:1:250~1:1000
IHC:1:20~1:100
ICC:1:100~1:200
ICC/IF:1:100~1:200
Notes:Optimal dilutions/concentrations should be determined by the researcher.
Additional Information
Form Ascitic fluid containing 0.03% sodium azide.
Storage Instructions 4℃; -20℃ for long term storage
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