Application
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);Application
Figure 1: Western blot analysis using HIF1A mouse mAb against Cos7 (1), Hela (2), Jurkat (3), RAJI (4) and NIH/3T3 (5) cell lysate.Application
Figure 2: Immunohistochemical analysis of paraffin-embedded liver cancer tissues (left) and lung cancer tissues (right) using HIF1A mouse mAb with DAB staining.Application
Figure 3: Immunohistochemical analysis of paraffin-embedded stomach cancer tissues (left) and brain tumor tissues (right) using HIF1A mouse mAb with DAB staining.Application
Figure 4: Immunofluorescence analysis of Hela cells using HIF1A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.| Product Name | HIF1A |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HIF1; MOP1; PASD8; bHLHe78; HIF-1alpha; HIF1-ALPHA; HIF1A |
| Immunogen | Purified recombinant fragment of human HIF1A expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Unknow |
| Tested Applications | WBIHCICCICC/IF |
| WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseMonkey |
| Alternative Names | HIF1 MOP1 PASD8 bHLHe78 HIF-1alpha HIF1-ALPHA HIF1A |
|---|---|
| Molecular Weight(MW) | 120kDa |
| Entrez Gene | 3091 |
|---|
Application
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Application
Figure 1: Western blot analysis using HIF1A mouse mAb against Cos7 (1), Hela (2), Jurkat (3), RAJI (4) and NIH/3T3 (5) cell lysate.
Application
Figure 2: Immunohistochemical analysis of paraffin-embedded liver cancer tissues (left) and lung cancer tissues (right) using HIF1A mouse mAb with DAB staining.
Application
Figure 3: Immunohistochemical analysis of paraffin-embedded stomach cancer tissues (left) and brain tumor tissues (right) using HIF1A mouse mAb with DAB staining.
Application
Figure 4: Immunofluorescence analysis of Hela cells using HIF1A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.| Application Notes | WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Ascitic fluid containing 0.03% sodium azide. |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
